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. 2022 Aug 10;13:4684. doi: 10.1038/s41467-022-32348-3

Fig. 2. Both EGFR and amphiregulin expression were selectively increased in ATMs in WT mice 6 weeks after the HFD.

Fig. 2

A HFD treatment led to selective increases in Egfr mRNA levels in epididymal fat tissue. N = 4 and 5. B HFD treatment also led to selective increases in EGFR ligand, Areg mRNA levels in epididymal fat tissue. N = 4 and 5. C Immunoblotting confirmed increased EF AREG in HFD-treated mice. D Representative photomicrographs of 3 independent experiments showed that both EGFR and AREG expression was minimal in mice with chow food but was evident and primarily colocalized with CD68 in ATMs. Scale bar = 100 μm. E In HFD-treated mice, AREG expression in EF adipocytes was minimal (representative of 3 independent experiments). Scale bar = 100 μm. Data are means ± SEM, ***P < 0.001, analyzed using 2-way ANOVA followed by Bonferroni’s post hoc test for A and B.