Fig. 3. Myeloid EGFR deficiency attenuated insulin resistance in diet-induced obesity.

WT (EGFR^f/f) mice and MΦ EGFR^−/− (CD11b-Cre; EGFR^f/f) mice were fed the HFD for 12 weeks. A Epididymal fat (EF) Egfr mRFNA levels were markedly increased in WT mice response to the HFD. MΦ EGFR^−/− mice had markedly lower EF Egfr mRNA levels compared to WT mice with chow food or with the HFD. N = 5. B Representative images showed that EGFR immunofluorescence was minimal in MΦ EGFR^−/− mice but clearly evident in ATMs in WT mice. Scale bar = 100 μm. C–F MΦ EGFR^−/− mice had reduced increases in body weight (n = 10 and 11) C, visceral and subcutaneous fat tissue (VAT and SAT) (n = 5) D fasting blood glucose (n = 9 and 11) E and HbA1c (n = 8 and 11) F. G, H MΦ EGFR^−/− mice exhibited improved glucose tolerance (n = 8 and 10) G and insulin tolerance (n = 9) H. Data are means ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, analyzed using 2 tailed Student’s t test for F; 2-way ANOVA followed by Tukey’s post hoc test for C, E, and H; 2-way ANOVA followed by Bonferroni’s post hoc test for A and D; and 2 tailed Student’s t test and 2-way ANOVA followed by Tukey’s post hoc test for G.