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. 2022 Aug 10;13:4684. doi: 10.1038/s41467-022-32348-3

Fig. 4. Mice with selective EGFR deletion in myeloid cells selectively attenuated white adipose tissue insulin resistance in diet-induced obesity.

Fig. 4

Hyperinsulinemic-euglycemic clamps were performed on 5 h fasted WT and MΦ EGFR−/− mice after 12 weeks on the HFD. A MΦ EGFR−/− mice had lower plasma insulin levels at baseline and during clamp periods. N = 5 and 7. B MΦ EGFR−/− mice had less severe insulin resistance, as more glucose infusion was needed to maintain a constant blood glucose. N = 5 and 7. C, D. MΦ EGFR−/− mice had increased rates of glucose disappearance (Rd) (N = 4 and 7) C and decreased endogenous glucose production (EGP) (N = 4 and 7) D. E MΦ EGFR−/− mice had increased glucose uptake, a marker of insulin resistance in VAT and SA. N = 5 and 7. F Representative images showed more insulin-stimulated p-Akt in EF in MΦ EGFR−/− mice. Scale bar = 100 μm. G Immunoblotting determined higher insulin-stimulated p-Akt in EF in MΦ EGFR−/− mice. N = 3 and 4). Data are means ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, analyzed using 2-way ANOVA followed by Bonferroni’s post hoc test for A, and C–E; 2-way ANOVA followed by Tukey’s post hoc test for B; and 2 tailed Student’s t test for G.