Fig. 6. Myeloid EGFR^−/− mice had less ATM accumulation and lower proinflammatory cytokines/chemokine in diet-induced obesity.

WT and MΦ EGFR^−/− mice were fed the HFD for 6 and 12 weeks. A MΦ EGFR^−/− mice had lower Emr1 (F4/80) (N = 5 and 8) and Cd68 (N = 5 and 8) expression in both EF and IF compared to WT mice at 12 weeks after the HFD. B Quantitative F4/80 staining demonstrated fewer ATMs and CLSs in both EF and IF in MΦ EGFR^−/− mice than WT mice at 12 weeks after the HFD. N = 8 and 10, scale bar = 200 μm. C Flow cytometry confirmed less EF CD45⁺CD11b⁺F4/80⁺ ATMs at both 6 and 12 weeks after the HFD in MΦ EGFR^−/− mice than WT mice. N = 4–6. D MΦ EGFR^−/− mice had lower EF mRNA expression of both proinflammatory cytokines (Nos2, Il1b, and Ccl3) and antiinflammatory cytokines (Cd206/Mrc1, Arg1, Il4ra, and Cd209) at 12 weeks on the HFD. N = 6. E, F Double immunofluorescent staining demonstrated fewer M1 ATMs (CD68⁺TNF⁺ and CD68⁺IL-1β⁺ positive cells) (N = 6) and M2 ATMs (CD68⁺CD206⁺ and CD68⁺Arg1⁺ positive cells) (N = 8) in EF in MΦ EGFR^−/− mice than WT mice. Scale bar = 100 μm. Data are means ± SEM, **P < 0.01, ***P < 0.001, analyzed using 2-way ANOVA followed by Bonferroni’s post hoc test for all.