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. 2022 Aug 10;13:4684. doi: 10.1038/s41467-022-32348-3

Fig. 9. Both intrinsic EGFR in bone marrow derived monocyte (BMDMs) and adipose tissue from MΦ EGFR−/− mice affected the recruitment of BMDMs in diet-induced obesity.

Fig. 9

WT and MΦ EGFR−/− mice were fed the HFD for 6 or 12 weeks and EF was used for experiment. A Schematic of experimental protocol. B WT BMDMs had greater EF infiltration at 6 weeks than at 12 weeks in WT recipient mice. Infiltration into EF was markedly decreased in MΦ EGFR−/− recipients at both time points. N = 4 and 5 and 6. C The infiltration of EGFR−/− BMDMs in EF was not increased after high fat diet in either WT or MΦ EGFR−/− recipients. N = 4 and 5 and 6. D MΦ EGFR−/− mouse epididymal fat tissue had lower mRNA expression of chemoattractants, including Ccl2, Cx3cl1, Cxcl1, Cxcl2, and Cxcl12. N = 4. E ATMs isolated from MΦ EGFR−/− mice had lower Cx3cr1 mRNA expression. N = 8. F High CX3CR1 expression in ATMs seen in WT mice was decreased in MΦ EGFR−/− mice 6 weeks after the HFD. Scale bar = 50 μm. Data are means ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, analyzed using 2-way ANOVA followed by Bonferroni’s post hoc test for all.