FIG. 4.

Primer extension analysis of dlt transcripts. A radiolabeled oligonucleotide was annealed to RNA isolated from S. mutans cultures grown in CDM supplemented with either glucose alone (Glu) or both fructose and glucose (Fru/Glu). The cDNAs resulting from the addition of avian myeloblastosis virus reverse transcriptase were run alongside the dideoxy sequencing reactions involving plasmid pYA3023. The arrows indicate the transcription start sites at guanine residues corresponding to nt 156 (left) and 203 (right).