TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| E. coli strains | ||
| HB101 | supE44 hsdS20 recA13 ara-14 proA2 lacY galK2 rpsL20 xyl-5 mtl-1 | 2 |
| JM109 | recA1 supE44 endA1 hsdR17 gyrA96 relA1 thiΔ(lac-proAB) | 33 |
| CLEO1 | HB101 transformed with pMC1; Tcr Cmr | This work |
| S. mutans strains | ||
| UA130 | Serotype c; IPS+ | P. Caufield |
| GMS100 | UA130 with dlt::cat fusion from pMC1; Tcr | This work |
| BM71 | PTS+ | 3, 16 |
| DC10 | ptsI::erm PTS− | 8 |
| Plasmids | ||
| pAM620 | pVA891::pAD1EcoRIF′::Tn916 Tcr Emr | 32 |
| pMH109 | Contains promoterless cat gene preceded by pUC13 MCS; Tcr | 12 |
| pNC10 | pMH109 derived; contains promoterless cat gene; Tcr ColE1 | This work |
| pNC12 | pNC10 derived; ColE1 tetM | This work |
| pMC1 | pNC12 derived; ColE1 dlt::cat tetM Cmr | This work |
| pDLT1-2 | 3.1-kb S. mutans amplicon containing dlt1 and dlt2 in pGEM-T Easy; Apr | This work |
| pDLT3 | 0.3-kb S. mutans amplicon containing dlt3 in pGEM-T Easy; Apr | This work |
| pDLT3-4 | 1.7-kb S. mutans amplicon containing dlt3 and dlt4 in pGEM-T Easy; Apr | This work |
| pYA3023 | 6.9-kb BglII fragment containing S. mutans dlt in pUC19; Apr | 28 |
| pYA3031 | 8-kb XbaI fragment containing S. mutans dlt in pUC19; Apr | This laboratory |