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Sperm parameters of the proband with several semen analyses
| Primer | Sequence | Tm | |
| ARMC2-F | 5ʹ-CAGGATTTAGTCGTCCGTGTTG -3ʹ | 58 °C | |
| ARMC2-R | 5ʹ-GGAAAGTTGGTTGTGTCTGCTG -3ʹ | ||
| Program | Cycles | Target (°C) | Hold (hh:mm:ss) |
| Initial-denature | 1 | 95 | 3 mm |
| Denature | 35 | 95 | 15 ss |
| Annealing | 55 | 15 ss | |
| Extension | 72 | 30 ss | |
| Final extension | 1 | 72 | 5 mm |
