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. 2022 Jul 28;13:927324. doi: 10.3389/fendo.2022.927324

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Copyright © 2022 Wang, Gao, Wang and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differentiation protocols of pancreatic β-cells from human iPSCs. Through mimicking the normal human pancreas development, directed differentiation protocols via adding different growth factors and small molecules can induce human iPSCs into pancreatic β-cells in vitro. This figure summarizes the 7-stage differentiation protocol and key markers of the differentiation pancreatic cells for evaluation of the consecutive stages of differentiation [(mainly based on references (20, 21)].