FIG. 3.
Comparison of the ςG consensus promoter sequence with the sleB promoter sequences of B. subtilis and B. cereus and slot blot analysis of sleB mRNAs from various ς factor-deficient strains of B. subtilis. (A) The consensus ςG promoter sequence in the −10 and −35 regions is from Corfe et al. (3). Boldface bases are conserved in >80% of all B. subtilis promoters transcribed by E-ςG. Underlined bases in the sleB promoters that differ from the consensus sequence are found at this position in at least one other B. subtilis ςG-dependent promoter. (B) Five micrograms of total RNA from B. subtilis 168 AJ12866 (wild type), 1S60 (spoIIG SigE−), 1S38 (spoIIIC SigK−), or OD8603 (OR3Δ SigG−) at t4 was applied per lane and hybridized with the same 32P-labeled sleB probe as used for Fig. 1A. Use of an increased amount (up to 50 μg per lane) of RNA or RNA from t5 or t6 gave the same results (data not shown).