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. 2022 May 19;23(9):1303–1319. doi: 10.1111/mpp.13230

FIGURE 5.

FIGURE 5

Relative expression of salicylic acid (SA) marker genes PR1a, PR1b, and WRKY45 in root tissues of rice plants treated with dehydroascorbate (DHA) or infected with Meloidogyne graminicola, analysed at (a) 1 and (b) 4 days after DHA treatment (DPT) and (c) 3 days after nematode inoculation (DPI) in mock‐treated and (d) 3 DPI in DHA‐treated plants. Expression levels were determined by reverse transcription‐quantitative PCR using three technical and three biological replicates, and are expressed relative to mock‐treated plants. Error bars indicate the 95% confidence interval. Gene expression levels were normalized using two internal reference genes and statistical analysis was done in REST 2009. Asterisks indicate significant differential expression in comparison with mock‐treated plants (*p < 0.05, ***p < 0.001).