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. 2022 Jul 28;26(3):290. doi: 10.3892/mmr.2022.12806

Figure 3.

Figure 3.

circ_0005699 sponges miR-450b-5b. (A) circ_0005699 and miR-450b-5p binding sites were determined using the starBase online database. (B) Results of dual-luciferase reporter gene assay. **P<0.01 vs. miR-NC. (C) Results of RNA pull-down assay. (D) RNA immunoprecipitation assay using AGO2, with IgG used as a control. **P<0.01 vs. IgG. (E) Relative expression of miR-450b-5p after circ_0005699-knockdown. **P<0.01 vs. sh-NC. miR, microRNA; Mut, mutant; NC, negative control; sh, short hairpin RNA; WT, wild-type.