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Effects of czcRS mutations on the concentration of czcCBA mRNA
| Bacterial strain | Genotype | cDNA concn (ng/g of RNA; mean ± SD [n = 2])
|
Fold induction | |
|---|---|---|---|---|
| Uninduced | Induceda | |||
| AE128(pMOL30) | Wild type | 11 ± 5 | 804 ± 42 | 73.7 |
| DN178(pMOL30-10) | ΔczcR | 5 ± 0 | 1,420 ± 270 | 313 |
| DN179(pMOL30-11) | ΔczcS | 40 ± 3 | 1,150 ± 20 | 28.9 |
Cells were induced for 10 min with 300 μM Zn2+ before the RNA was isolated.
