Figure 3.
(A) Cellular uptake (scale bar = 100 μm). (B) Cytotoxicity of B16F10 cells. (C) Cytotoxicity of B16F10 cells with 808 nm laser irradiation (1.5 W/cm2, 2 min). (D) Calcein-AM/PI staining (scale bar = 100 μm). (E) Surface CRT expression. (F) Extracellular release of HMGB1. (G) The quantitative analysis of DCs uptake of FITC-OVA. (H) Lysosomal escape test (scale bar = 10 μm). (I) DCs maturation measurement. (J) FRI of mice received different formulations. (K) FRI of LNs harvested from mice at 36 h. (L) Quantification of fluorescence intensity in axillary and inguinal LNs. The results of significant difference analysis were compared with FA-TSL/AuNCs/IR780 + laser group. The data are presented as mean ± SD (n = 3). ∗∗∗P < 0.001.