Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 28;9:940395. doi: 10.3389/fvets.2022.940395

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Ho, Trinh, Tran, Le, Nguyen, Hoang, Pham, Conrad, Pham and Chu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Humoral immune responses and cytokine responses in pregnant sows. Data are presented as mean ± standard deviation (SD). * indicates a statistically significant difference (p < 0.05). (A) Immunization and challenge experiment scheme in pregnant sows (~80 days of gestation, n = 1 per group) and piglets (n = 8 for COE/G2a-pII group and n = 2 for PBS group). The green arrow and red arrow indicate immunization and bleeding time, respectively. (B) PEDV-specific IgG antibodies in sow sera vaccinated with COE/G2a-pII or PBS were measured by a commercial ELISA kit. An S/P ratio > 0.35 was defined as positive with PEDV-specific IgG antibody. (C) COE-specific IgA antibodies in sow sera were evaluated by an ELISA using SEC-purified COE/G2a-pII as antigen. (D) Neutralizing antibodies in sow sera were analyzed by the virus-neutralizing assay. The highly virulent NAVET/PEDV/PS6/2010 strain (200 TCID50/0.1 ml) was used for the assay. A VN titer ≥ 8 was defined as positive with neutralizing antibody against PEDV. (E) IFN-γ levels in sow sera were measured based on the standard curve of recombinant porcine IFN- γ standard in a commercial ELISA kit.