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. 2022 Jul 28;9:940395. doi: 10.3389/fvets.2022.940395

Figure 3.

Figure 3

Passive transfer of antibodies and cytokine from sows to piglets at the days 0 and 10 pc. Data are presented as mean ± standard deviation (SD). * indicates a statistically significant difference (p < 0.05). (A) PEDV-specific IgG antibodies in piglet sera from sows (n = 1 per group) vaccinated with COE/G2a-pII, or PBS were measured by a commercial ELISA kit. An S/P ratio > 0.35 was defined as positive with a PEDV-specific IgG antibody. (B) COE-specific IgA antibodies in piglet sera (n = 8 for COE/G2a-pII group and n = 2 for PBS group) were evaluated by an ELISA using SEC-purified COE/G2a-pII as antigen. (C) Neutralizing antibodies in piglet sera were analyzed by the virus-neutralizing assay. The highly virulent NAVET/PEDV/PS6/2010 strain (200 TCID50/0.1 ml) was used for the assay. A VN titer ≥ 8 was defined as positive with neutralizing antibody against PEDV. (D) IFN-γ levels in piglet sera were measured based on the standard curve of recombinant porcine IFN-γ standard in a commercial ELISA kit.