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Effect of cholate on the growth of wild-type (wt) B. subtilis and several uncomplemented and complemented strains with mutations in the mrp gene. Cells were grown in TKM medium (pH 7.0) in the presence (hatched bars) or absence (open bars) of 0.08% (wt/vol) cholate. The absorbance at 600 nm (A600) was determined after 6 h of shaking at 30°C. The results represent the mean of at least six determinations; standard deviations are shown as error bars.
