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. 2022 Jul 28;9:974759. doi: 10.3389/fcvm.2022.974759

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Copyright © 2022 Shi, Zhao, Lyubenov, Motrapu, Li, Steiger, Mammadova-Bach, Yang, Liu and Anders.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The circadian oscillations of neutrophils, and chemokine in mouse blood in a healthy state. (A) Flow cytometric quantification of neutrophils baseline in the blood. (B,C) MFI of surface CXCR4 (B, n = 5 mice/group) and CXCR2 (C, n = 5 mice/group) expression by neutrophils in the blood as determined by flow cytometry. (D,E) Plasma levels of CXCL12 (D, n = 5 mice/group) and CXCL1 (E, n = 5 mice/group) in healthy mice as determined by ELISA. Data are means ± SD. MFI: mean fluorescence intensity; CXCR2, CXC chemokine receptor 2; CXCR4, CXC chemokine receptor 4; CXCL12, CXC motif chemokine ligand 12.