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Influence of HOQNO on fumarate reduction to succinate by intact cells (A and B) or purified membranes (C and D) from A. succinogenes under 2 atm of H2 (A and C) or with electrically reduced NR (B and D). Whole cells (OD660, 4.2) and purified membranes (2.65 mg of protein/ml) were suspended in 50 μM KPO4 buffer (pH 7.2), and where indicated, 50 mM NR was added. Solid symbols, without HOQNO; open symbols, with addition of 2 μM HOQNO.
