Figure 1.

Overexpression of miR-615-3p alleviates apoptosis and oxidative stress. (A) The expression level of miR-615-3p in H/R-stimulated HCMs was examined by RT-qPCR. (B) Overexpression efficiency of miR-615-3p was detected by RT-qPCR. (C) Cell viability of HCMs in control, H/R, H/R+NC mimics, and H/R+miR-615-3p mimics group was confirmed by CCK-8. (D-E) Cell apoptosis rates of HCMs in different groups were detected by flow cytometry. (F) ROS levels in HCMs in different groups. (G-H) The changes in MDA concentration and SOD enzymic activity in HCMs were detected by corresponding commercial kits. (I) ATP levels in HCMs in different groups were measured. *P < .05, **P < .01, ***P < .001. HCMs, human cardiomyocytes; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; ROS, reactive oxygen species.