Figure 2.

MEF2A is a target gene of miR-615-3p. (A) The expression levels of the six target genes (PSMD11, ZNF626, ITSN1, MEF2A, USP44, and TOMM7) in HCMs were examined by RT-qPCR. (B) Western blot was conducted to examine the protein level of MEF2A in H/R-stimulated HCMs. (C) Targetscan predicted the binding sites between miR-615-3p and MEF2A 3’-UTR. (D) Schematic diagram of the pmiR-RB-Report-MEF2A-3'UTR-WT/MUT vector. (E) Luciferase activities of HCMs transfected with luciferase reporter vectors and miR-615-3p mimics were assessed. (F-G) The mRNA expression and protein levels of MEF2A in HCMs transfected with miR-615-3p mimics were detected by RT-qPCR and western blot, respectively; **P < .01, ***P < .001. HCMs, human cardiomyocytes; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; H/R, hypoxia/reoxygenation; UTR, untranslated region.