Figure 3.

Overexpressed MEF2A reverses the inhibitory effects of miR-615-3p overexpression on oxidative stress and apoptosis. (A-B) The mRNA expression and protein levels of MEF2A in HCMs transfected with NC mimics, miR-615-3p mimics, or cotransfected with miR-615-3p mimics+pcDNA3.1-MEF2A were examined by RT-qPCR and Western blot, respectively. (C) CCK-8 assay showed the cell viability of HCMs in three groups. (D-E) Flow cytometry was conducted to examine the apoptosis rates of HCMs transfected with NC mimics, miR-615-3p mimics, or cotransfected with miR-615-3p mimics+pcDNA3.1/MEF2A. (F) H2DCF-DA staining was used to detect ROS accumulation. (G-H) The rescue effects of MEF2A on miR-615-3p in MDA concentration and SOD enzymic activity were measured. (I) The rescue effect of MEF2A overexpression on miR-615-3p in ATP level was measured. *P < .05, **P < .01, ***P < .001. HCMs, human cardiomyocytes; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; ROS, reactive oxygen species.