Fig. 3.

Double-immunohistochemical staining with PAX5 (nucleus, brown) and CD20 (cytoplasm, magenta) in early-stage (ES) mycosis fungoides (MF), advanced-stage (AS) MF, dermatitis (D) and healthy skin (HS). Presence of double-immunostained small-to-medium-sized B-cells in 3 different patients with (A–C) ES-MF, (E–G) AS-MF, (I–K) D and (M–O) HS. (E) PAX5⁺/CD20⁺ large, atypical, centroblast-like B-cells in a patient with AS (non-transformed MF). (A–C, E–G, I–K) Original magnification ×100, (E) white dotted line higher power field (×600) of the double-immunostained B-cells. Higher power field (×200) of patients (D) A, (H) G, (L) K, and (P) M.