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. 2022 Jul 28;13:944388. doi: 10.3389/fimmu.2022.944388

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Copyright © 2022 Li, Jiang, Zhang, Huang, Xu, Chen, Hou, Cai, Huang and Jian

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Relative expression levels of On-TPH1, On-TPH2, On-SERT, On-MAO, and On-HTRs mRNA among different tissues in healthy Nile tilapia, assessed via qRT-PCR. n = 3. Expression level of each gene in the brain was set as 1000. (B) Western blot analysis of On-HTR1A and On-TPH2 in different tissues of healthy Nile tilapia. GAPDH was applied as a reference protein. See Figure S2 for the complete figures. (C) Location of 5-HT and On-HTR1A in the Nile tilapia brain. Top, diagram of Nile tilapia brain morphology. Left column, diagram of sections with typical brain structures in healthy Nile tilapia. Midcolumn, H&E of each selected section. Right column, On-HTR1A (green), 5-HT (red), and nucleus (blue) of each section determined via immunofluorescence (IF).