Figure 7.

WS-716 enhanced the apoptosis induced by paclitaxel (PTX) through the mitochondrial pathway in SW620/Ad300 cells. SW620/Ad300 cells were treated with WS-716 (20 μmol/L, 4 h), PTX (1 μmol/L, 2 h）or combination of WS-716 and PTX. (A) Damaged cell nucleus of SW620/Ad300 was detected by Hoechst 33258 staining. The scale bar is 100 μm. (B) Apoptosis was measured by FCM. (C) Expression of cleaved caspase-9, -3, -7, PARP, NLRP3, cleaved caspase-1, Gasdermin D, and LC3B was measured by Western blot. (D) The mitochondrial membrane potential was evaluated by FCM. (E) Bax, Bcl-2, Bcl-xL, and cytochrome c in cytoplasm expression were analyzed by Western blot at 24 h. β-Actin was used as a loading control. Data are represented as mean ± SD, n = 3. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 vs. NC, ^#P < 0.05.