Figure 1.

Characterization of P-Lipo. (A) Schematic showing the preparation of P-Lipo. (B) Transmission electron microscopy and cryo-electron microscopy analysis revealed a spherical homogeneous shape for bare liposomes and P-Lipo after extrusion (scale bar = 100 nm). (C) The fluorescence spectra of P-Lipo when different amounts of platelet membranes were input. C6-stained lipid membranes and RhB-labeled platelet membranes were fused to form P-Lipo. (D) Representative super-resolution fluorescence images showed the colocalization of DiD-labeled lipid membranes and DiO-labeled platelet membranes after fusion together. Scale bar = 20 μm. Fluorescence intensity traces (dotted yellow lines) from the left image were plotted at the right. Peak overlapping indicated the colocalization of artificial lipid membranes and platelet membranes. (E) Representative confocal laser scanning microscopy (CLSM) images of single 4T1 cell after incubation with P-Lipo (scale bar = 5 μm). Cell nuclei, artificial lipid membranes, and platelet membranes were labeled with DAPI (blue), DiO (pinkish-red), and DiO (green), respectively. Fluorescence intensity traces (dotted yellow line) from the left image were plotted at the right. Dynamic light scattering analysis displayed (F) the average diameter, (F) polydispersity index (PDI), and (H) zeta potential of Lipo, P^L-Lipo, and P^H-Lipo (n = 3). (I) Size stability of P-Lipo over 7 days in PBS at 4 °C (n = 3).