Figure 2.

Characterization of platelet membrane proteins in P-Lipo. (A) Coomassie staining and (B) Western blot analysis of protein markers (0), Lipo (1), P^L-Lipo (2), P^H-Lipo (3), platelet membranes (4), platelet supernatant (5), and platelets (6) after SDS-PAGE separation. (C) Transmission electron microscopy images of P-Lipo probed with anti-CD61-gold, followed by negative staining with 2% uranyl acetate (scale bar = 50 nm). (D) Flow cytometry histograms of CD42b expression on the surface of P-Lipo. Unstained Lipo functioned as a negative control. (E) Venn diagram of identified proteins of P-Lipo and platelet membranes (PM) analyzed by liquid chromatography-tandem mass spectrometry. (F) Venn diagram of identified proteins extracted from P-Lipo by cellular component. (G) Functional characterization of the platelet membrane proteins identified in P-Lipo by biological process. (H) A heat map depicting the typical membrane protein levels (normalized to array reference) from P-Lipo and PM.