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. 2022 Jan 12;12(8):3427–3447. doi: 10.1016/j.apsb.2022.01.005

Figure 3.

Figure 3

In vitro CTC targeting of P-Lipo. (A) CLSM images of 4T1-GFP cells (green) captured by DiD-labeled P-Lipo (red) in the artificial CTCs-containing blood. (B) Scanning electron microscopy images of P-Lipo attached to the surface of 4T1 cell (scale bar = 5 μm). The yellow arrows indicated P-Lipo (or Lipo) adherent on the cell surface. (C) Flow cytometry histograms and (D) the corresponding fluorescence intensity of 4T1-GFP captured by DiD-labeled P-Lipo (or Lipo) from the artificial CTC-containing blood (n = 3). Untreated CTC-containing blood served as a control. ∗∗P < 0.01. (E) Flow cytometry histograms of 4T1-GFP in the artificial CTC-containing blood captured by DiD-labeled PH-Lipo, which were preincubated with heparin or fucoidin. Untreated CTC-containing blood served as a control.