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. 2022 Jan 12;12(8):3427–3447. doi: 10.1016/j.apsb.2022.01.005

Figure 4.

Figure 4

In vivo CTC targeting of P-Lipo. (A) Schematic illustration of the two-color, two-slit in vivo flow cytometer. (B) Visualization of digitized fluorescence signals of 4T1 cells (Green line) and DiD-labeled P-Lipo (or Lipo) (red line) by in vivo flow cytometry (IVFC). Each peak was created by the fluorescence burst resulting from tumor cells or P-Lipo traversing the excitation slit. Individual fluorescence peaks were shown in more detail on the right. (C) CTC capture efficiency in vivo (n = 3). The bar chart revealed the number of CTC peaks and captured CTC peaks, while the point plot depicted the percentage of dual-positive peaks in CTC peaks. ∗∗P < 0.01. (D) The images of CTC (green) captured by DiD-labeled P-Lipo or Lipo (Red) in the mesenteric vessels visualized by the spinning-disk confocal microscope (scale bar = 200 μm) and the corresponding fluorescence intensity profiles of P-Lipo or Lipo (red) and 4T1 cells (green) along the dotted yellow line crossing the representative cells.