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. 2022 Jan 12;12(8):3427–3447. doi: 10.1016/j.apsb.2022.01.005

Figure 5.

Figure 5

In vivo targeting of P-Lipo in lung pre-metastasis. (A) Representative bioluminescence imaging (left) and fluorescence imaging (right) at 2 h after intravenous injection of DiD-labeled P-Lipo (or Lipo) in the pre-metastasis model. Ex vivo imaging of major organs (from top to down: heart, liver, spleen, lung, kidney, and brain) was to evaluate the distribution of P-Lipo (or Lipo) after injection. (B) Bioluminescence intensity and fluorescence intensity of major organs to reveal the distribution of P-Lipo (or Lipo) (n = 3). ∗P < 0.05, ∗∗P < 0.01. (C) Representative fluorescence images of 4T1-GFP cells colocalized with DiD-labeled P-Lipo (or Lipo) in the lung at 20 min and 24 h after intravenous injection and the corresponding fluorescence intensity profiles of yellow dotted lines shown on the right. Scale bar = 100 μm. Blue, nuclei; green, 4T1-GFP cells; red, vessels stained by rhodamine-conjugated dextran; rosy, DiD-labeled P-Lipo (or Lipo).