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. 2022 Jan 12;12(8):3427–3447. doi: 10.1016/j.apsb.2022.01.005

Figure 7.

Figure 7

Anti-tumor metastasis efficacy of P-Lipo. (A) Representative flow cytometry scatter plot of 4T1-GFP in the peripheral blood cells from heath mouse. (B) Flow cytometry scatter plot of peripheral blood cells from the pre-metastasis mouse models on Day 3 after different treatments. (C) Quantification of 4T1-GFP cells in the peripheral blood using the flow cytometry (n = 4). (D) Bioluminescence imaging of the pre-metastasis mouse models at preset time points after different treatments. (E) Quantification of bioluminescence intensity of the pre-metastasis mouse models over 15 days after treatment (n = 4). (F) Representative hematoxylin-eosin (H&E)-staining images of the lung sections from different treatment groups (scale bar = 2 mm). Arrowheads indicated tumor areas or nodules. (G) Representative images of Ki67-stained lung slices from different treatment groups (scale bar = 50 μm). Ki67 positive cell nuclei were always stained by diaminobenzidine and appeared as brown, while normal cell nuclei were stained by hematoxylin. (H) The area of tumor metastasis in H&E-staining lung sections from different treatment groups (n = 3). (I) Numbers of the lung metastatic nodule after different treatments (n = 3). (J) Percentage of Ki67-positive cells in lung sections from different treatment groups (n = 4). ∗P < 0.05, and ∗∗P < 0.01.