P-Lipo regulated the immune cell expression in the blood of the pre-metastasis mouse models (n = 3). (A) Representative flow cytometry analysis of CD4+ T cells, CD8+ T cells, MDSC (CD11b+Gr1+), M1 macrophages (F4/80+CD86+), and M2 macrophages (F4/80+CD206+) in the blood (gated on CD45+ cells) of the pre-metastatic mouse models after different treatments. (B) Percentage of CD4+ T cells, CD8+ T cells, CD4+/CD8+ T cell ratio, and myeloid-derived suppressor cells (MDSC), M1 macrophages, M2 macrophages in the blood of the pre-metastasis mouse models after different treatments. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 compared with PBS. ΔP < 0.05 compared with Lipo. (C) White blood cells (WBC), lymphocyte (Lymph), monocyte (Mon), granulocyte (Gran), hemoglobin (HGB), and platelet (PLT) levels in the blood of the pre-metastasis mouse models at 15 days after different treatments. Dotted lines indicated the normal range. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.