Fig. 3.

IκBζ is involved in tumour necrosis factor-α (TNFα)/interleukin (IL)-17A-induced IL36G mRNA expression. Human keratinocytes were transfected with IκBζ or control siRNA (siCon) 24 h before stimulation with TNFα or/and IL-17A for (a and b) 1 h or (c) 24 h. IκBζ knockdown was measured by (a) western blotting (n = 6) using β-actin as a loading control or by (b) qPCR (n = 4) using RPLP0 as a reference gene. (c) qPCR was used to analyse IL36G mRNA expression (n = 6). RPLP0 was used as a reference gene for normalization. Results from qPCR are expressed as mean ± SD. *p < 0.05 compared with the corresponding siCon samples.