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. 2022 Jul 28;15:908911. doi: 10.3389/fnmol.2022.908911

FIGURE 7.

FIGURE 7

Knockdown of Netrin-1 (NTN-1) in hippocampal CA1 regions decreased not only visceral sensitivity but also the expression levels of mRNA and proteins of Netrin-1, DCC, GluA1, and PSD95. (A) The representative external abdominal oblique muscle EMG recordings. (B) VMR amplitudes to 40 and 60 mmHg CRD were significantly reduced in rats expressing Lenti-shNTN-1 (MS + shNTN-1) than in rats injected Lenti-Scramble (MS + Scramble). MS + Scramble: n = 12. MS + shNTN-1: n = 12. All data were given as mean ± SEM. ***P < 0.001, two-tailed unpaired Student’s t-test. (C) The mRNA and (E) protein expressions of Netrin-1, DCC, GluA1, and PSD95 in the hippocampus were significantly reduced in the MS + shNTN-1 group. (D) Representative immunoblots showed the expression of Netrin-1, DCC, GluA1, NR2A, NR2B, PSD95, and β-Tubulin. (F) A representative fluorescence image confirmed the successful GFP expression in shNTN-1 infected cells in the hippocampus. Scale bar, 500 μm. MS + Scramble: n = 4. MS + shNTN1: n = 4. All data were given as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed unpaired Student’s t-test.