TABLE 1.
Parameters of binding of OlpB1391-1664, SlpA27-235, EA132-213, and Sap32-211 polypeptides to C. thermocellum and B. anthracis cell walls
Protein |
C. thermocellum cell walls
|
B. anthracis cell walls
|
||
---|---|---|---|---|
KD (M) | Capacitya | KD (M) | Capacity | |
OlpB1391-1664 | 4.8 × 10−7 ([2.7–6.9] × 10−7b) | 66 (55–78b) | 1.9 × 10−7 ([1.2–2.6] × 10−7) | 18 (16–19) |
SlpA27-235 | 2.1 × 10−7 ([1.2–2.9] × 10−7) | 66 (58–74) | 7.1 × 10−7 ([3.7–10.4] × 10−7) | 24 (20–27) |
EA132-213 | 1.8 × 10−8 ([1.0–2.6] × 10−8) | 88 (83–93) | 1.8 × 10−7 ([0.9–2.6] × 10−7) | 46 (40–51) |
Sap32-211 | 3.1 × 10−8 ([1.7–4.6] × 10−8) | 91 (85–97) | 1.0 × 10−7 ([0.5–1.5] × 10−7) | 42 (36–48) |
Binding capacity of cell walls, expressed as millimoles of bound SLH domains per mole of meso-diaminopimelate (dap).
95% confidence interval.