TABLE 3.
ArgR- and ANR-dependent induction of the arc operon
| Strain | Plasmid | ADI sp act (U/mg)a
|
|
|---|---|---|---|
| Without l-arginine | With 25 mM l-arginine | ||
| PAO1 | None | 11 ± 2 | 43 ± 4 |
| pME3731 | 343 ± 43 | 548 ± 99 | |
| pME3731-1 | 357 ± 82 | 351 ± 42 | |
| PAO501 (argR) | None | 9 ± 2 | 12 ± 2 |
| pME3731 | 287 ± 47 | 365 ± 63 | |
| pME3731-1 | 263 ± 27 | 318 ± 21 | |
| PAO6261 (anr) | None | 1 ± 1 | 1 ± 1 |
| pME3731 | 33 ± 3 | 31 ± 4 | |
| pME3731-1 | 66 ± 17 | 67 ± 11 | |
a
Cells were grown at 37°C under oxygen-limiting conditions in glutamate minimal medium with or without an arginine supplement to a final density of about 5 × 108 cells per ml. Specific activities are mean values ± standard deviations from six experiments.