Table 2.
Individual standard deviation and accumulated standard deviation for consecutive microRNA reference genes
miRNA
|
SD
|
SDacc
|
hsa-let-7i-5p | 0.49 | 0.49 |
hsa-miR-222-3p | 0.57 | 0.37 |
hsa-miR-23a-3p | 0.63 | 0.33 |
hsa-miR-30e-5p | 0.65 | 0.29 |
hsa-miR-191-5p | 0.79 | 0.28 |
The lower the standard deviation/accumulated standard deviation, the more applicable microRNA/microRNA combination was as reference gene. These miRNAs were used as endogenous controls for the normalization of real-time quantitative PCR data. SD: Standard deviation; SDacc: Accumulated standard deviation; miRNA: MicroRNA; miR: MicroRNA.