Figure 7.

Rescue of U87 cell migration. (a) Cell migration at 0, 6, and 20 h of untreated U87 cells, U87 treated with NTERA2 large extracellular vesicles (lEVs) or NTERA2-lEVs previously incubated with anti-CRIPTO antibody (lEVs-α-Cripto) and a scramble antibody (lEVs-α-IgG), respectively. Open area is outlined by a yellow line and analyzed with ImageJ. (b) Relative wound healing area in the different conditions. Three independent experiments were performed, each in duplicate. Data are shown as means ± standard error of mean. Inhibitory effect of NTERA2-lEVs was partially rescued after 20 h when vesicles were pretreated with anti-CRIPTO antibody. Rescue was not observed when anti-IgG antibody was added. * p < 0.05; ** p< 0.01; *** p < 0.001.