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Exosomes from the HCC827 cell line induced hybrid EMT. Quantification analysis of NFR2 (A) and P-cadherin (B) expression obtained by RT-qPCR of protein extracts of A549 cells treated with TGFβ and exosomes isolated from HCC827 supernatant (mean ± SEM, n = 3; statistical analysis was performed using unpaired t-test). Significance was set at * p < 0.05. (C) EMT score calculated by using RT-qPCR levels of two epithelial and three mesenchymal markers. (Chae et al., modified formula).
