Figure 4.

Akt pathway activity and EMT process in adenocarcinoma (ADC) and pulmonary sarcomatoid carcinoma (PSC) arisen in DKO mice. (A) Sections from lungs (upper panel), ADC (middle panel) and PSC (lower panel) were immunostained for p-Akt, mTOR, p70S6K and pS6, as indicated. Bars = 50 µm. (B) p-AKT and AKT were assessed using Western blotting analyses. Original image of western blot can be found at File S1 (C) Immunohistochemical analyses for the quoted proteins (E-cadherin, Vimentin and Snail) involved in the EMT processes in lungs (left column), ADC (middle column) and PSC (right column). Bars = 50 µm. (D) qRT-PCR analysis of Cdh1, Vim, Snai1 and Snai2 (encoding for E-cadherin, Vimentin, Snail and Slug, respectively) expression levels in ADC, PSC and control lungs. * p < 0.05, determined by Mann–Whitney.