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. 2022 Jul 31;14(15):3732. doi: 10.3390/cancers14153732

Figure 4.

Figure 4

High expression of hnRNPU in breast cancer and mapping of molecular interaction between CDC20 and hnRNPU. (a) The expression of hnRNPU was analyzed by Western blot in the normal breast epithelial cell line (MCF-10A), the ductal carcinoma in situ (DCIS) cell line, and different breast cancer cell lines. (b) The correlation between CDC20 and hnRNPU expression in breast cancer patients was analyzed by using correlationAnalyzerR. (c) Box plots showing relative expression of hnRNPU mRNA in human breast cancer and normal tissues. (d) Box plots showing relative expression of hnRNPU protein in human breast cancer and normal tissues. The transcriptome and proteome data were obtained from TCGA and CPTAC databases, respectively, and box plots were generated using the UALCAN web resource. (e) Kaplan–Meier overall survival (OS) curves of breast cancer patients with high and low hnRNPU expression levels. (f) Schematic representation of human hnRNPU with previously identified domains and deletion constructs. (g) Schematic representation of human CDC20 with previously identified domains. (h) 293T cells were transfected with equal amounts of the indicated plasmids. After transfection for 48 h, cells were harvested, and the cell lysates were immunoprecipitated with an anti-Myc antibody. Immunoblot analysis revealed that the amino acid region 461–653 on hnRNPU is required for its interaction with CDC20.