Table 3.
The elution gradient of the mobile phase.
| Time (min) | Flow Rate (mL/min) | A (%) | B (%) |
|---|---|---|---|
| 0 | 0.4 | 100 | 0 |
| 3.5 | 0.4 | 75.5 | 24.5 |
| 5 | 0.4 | 35 | 65 |
| 5.5 | 0.4 | 0 | 100 |
| 7.4 | 0.6 | 0 | 100 |
| 7.6 | 0.6 | 48.5 | 51.5 |
| 7.8 | 0.5 | 100 | 0 |
| 9 | 0.4 | 100 | 0 |
| 10 | 0.4 | 100 | 0 |
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