Table 6.

Effects of long-term protein restriction on fatty acid composition in longissimus thoracis muscle of Shaziling pigs.

Item	Dietary Protein Levels					SEM	p-Value
	+20%	10%	0	−10%	−20%		ANOVA	Linear	Quadratic
C10:0	0.09	0.09	0.09	0.09	0.08	0.00002	0.25	0.17	0.08
C12:0	0.07 A	0.08 A	0.08 A	0.07 AB	0.06 B	0.00002	0.01	0.06	<0.01
C14:0	1.51 a	1.31 bc	1.35 bc	1.41 b	1.23 c	0.00026	<0.01	<0.01	0.03
C16:0	28.84	28.36	28.71	28.82	28.16	0.00152	0.68	0.42	0.65
C16:1	3.12	2.88	2.68	3.01	2.79	0.00072	0.06	0.28	0.37
C17:0	0.17 A	0.17 A	0.17 A	0.16 AB	0.15 B	0.00003	0.02	<0.01	<0.01
C18:0	15.73	16.08	17.37	16.67	16.75	0.00193	0.08	<0.05	0.04
C18:1n9t	0.14 A	0.14 AB	0.12 B	0.14 AB	0.13 AB	0.00002	<0.05	0.05	0.06
C18:1n9c	40.66	38.87	38.37	39.87	39.07	0.00452	0.54	0.48	0.46
C18:2n6c	6.41	7.92	7.57	6.64	7.5	0.00317	0.55	0.67	0.72
C20:0	0.27	0.27	0.29	0.25	0.29	0.00007	0.38	0.68	0.84
C20:1	0.82	0.85	0.76	0.82	0.73	0.00017	0.19	0.10	0.21
C18:3n3	0.23 A	0.23 A	0.20 AB	0.20 AB	0.17 B	0.00006	<0.01	<0.01	<0.01
C20:2	0.23	0.26	0.23	0.22	0.23	0.00007	0.29	0.40	0.55
C20:3n6	0.19	0.22	0.28	0.21	0.24	0.00013	0.27	0.44	0.39
C20:4n6	1.31 b	2.21 ab	2.29 a	1.48 b	2.02 ab	0.00143	<0.05	0.48	0.33
SFA 1	46.69	46.36	48.06	47.48	46.72	0.00216	0.23	0.31	0.09
MUFA 2	44.74	42.74	41.94	43.84	42.72	0.00505	0.34	0.24	0.20
PUFA 3	8.37	10.85	10.57	8.74	10.15	0.00469	0.39	0.56	0.47
Σn6 PUFA 4	7.91	10.35	10.14	8.33	9.75	0.00462	0.33	0.52	0.46
Σn3 PUFA 5	0.23 A	0.23 A	0.20 AB	0.20 AB	0.17 B	0.00006	<0.01	<0.01	<0.01

SFA ¹ = C10:0 + C12:0 + C14:0 + C16:0 + C17:0 + C18:0 + C20:0. MUFA ² = C16:1 + C18:1n9t + C18:1n9c + C20:1 + C22:1n9. PUFA ³ = C18:2n6c + C18:3n3 + C20:2 + C20:3n6 + C20:4n6. n6 PUFA ⁴ = C18:2n6c + C20:3n6 + C20:4n6. n3 PUFA ⁵ = C18:3n3. ^A,B Values (n = 6–8) within a row with different capital superscripts differ significantly (p < 0.05) by Duncan’s multiple comparisons. ^a,b,c Values (n = 6–8) within a row with different lowercase differ significantly (p < 0.05) by the Kruskal–Wallis test.