FIG. 1.

Diagram of pHP8080. pHP8080 is a derivative of pHP808 (28, 30); pHP808, a pACYC184 derivative, contains the H. pylori urease gene cluster (ureABIEFGH) cloned into the BamHI site through a Sau3AI partial digestion of H. pylori 43504 genomic DNA (restriction site designated as B/S). The nixA nickel transporter gene was PCR amplified from pUEF201 (43) and cloned into the NruI and AvaI sites in pHP808, which removed the 3′ end of the tetracycline resistance marker. glmM (11) and lspA (58) refer to genes previously designated as ureC and ureD, respectively (10, 35) and are not required for urease activity (10, 44). Thick lines, pACYC184 vector sequences; cat, chloramphenicol resistance marker; tet′, truncated tetracycline resistance marker. Arrows show directions of transcription.