FIG. 2.

Schematic maps of pUDF104 and pBS-flbA. The numbering of the ORFs of the genes in pUDF104 is based on the system developed by Tomb et al. (58). The size of each complete gene and the size of the total insert are shown. The flbA gene plus flanking DNA was subcloned into the BamHI (B) and ClaI (C) sites in pBluescript by PCR as described in the Materials and Methods section to yield pBS-flbA. Truncated genes are denoted by jagged edges. H, HindIII; X, XbaI; N, NheI. Thick lines, pBluescript vector sequences. Arrows show directions of transcription.