TABLE 1.
Summary of UEFs in DH5α (pHP8080)
| Plasmid name | Insert size (kb)a | Candidate gene(s) | Mean urease activity ± SDb | nc | P valued | No. of similar clones |
|---|---|---|---|---|---|---|
| pBS | None | None | 778 ± 628 | 33 | NAe | NA |
| pUEF1004 | 2.7 | helAB, lpp | 6,518 ± 2,993 | 16 | <0.0001 | 21f |
| pUEF1004-511 | 0.4 | lpp | 2,505 ± 428 | 8 | 0.0003 | NA |
| pUEF1004-548 | 1.1 | helAB | 2,598 ± 838 | 9 | <0.0001 | NA |
| pUEF1004-548ΔK | 0.7 | helB | 4,161 ± 816 | 7 | 0.0001 | NA |
| pUEF1014 | 7.1 | hp0839–0846 | 2,917 ± 2,000 | 7 | 0.0335 | 1 |
| pUEF1023w | 8.4 | hp1400–1403 | 10,236 ± 4,449 | 10 | <0.0001 | 1 |
Size was determined based on the results of restriction analysis, PCR, and sequencing.
Urease activity in DH5α (pHP8080) plus each of the plasmids listed. Data are expressed as specific activity (nanomoles of NH3/minute/milligram of protein) (mean ± standard deviation).
n, number of experiments.
P value for urease activity as compared with urease activity from DH5α (pHP8080/pBluescript).
NA, not applicable.
Other clones determined to contain helAB (hp0548) and lpp (hp0511) based on the results of restriction analysis, PCR, and sequencing were as follows: pUEF1002, -1005, -1006, -1007, -1008, -1010, -1012, -1015, -1018, -1020, -1026, -1027, -1028, -1030, -1031, -1033, -1034, -1035, -1036, and -1038. Insert sizes varied from 2.7 to 12.3 kb.