TABLE 2.

Summary of UDFs in SE5000 (pHP8080)

Plasmid, extract	Insert size (kb)a	Candidate gene(s)	Mean urease activity ± SDb	nc	P valued	No. of similar clonese
pBS, cytosolicf	None	None	1,542 ± 786	41	NAg	NA
pBS, pellet	None	None	409 ± 203	5	NA	NA
pBS, supernatant	None	None	0 ± 0	3	NA	NA
pUDF104, cytosolic	4.7	hp1038–1042	450 ± 219	10	<0.0001	11
pBS-flbA, cytosolic	2.4	flbA	95 ± 73	11	<0.0001	NA
pBS-flbA, pellet	2.4	flbA	27 ± 19	5	<0.0001	NA
pBS-flbA, supernatant	2.4	flbA	0 ± 0	3	<0.0001	NA
pUDF140, cytosolic	4.0	hp554–558	0 ± 0	8	<0.0001	1

^aSize was determined based on the results of restriction analysis, PCR, and sequencing. 

^bUrease activity in SE5000 (pHP8080) plus each of the plasmids listed. Data are expressed as specific activity (nanomoles of NH₃/minute/milligram of protein) (mean ± standard deviation). 

^cn, number of experiments. 

^dP value for urease activity as compared with urease activity from SE5000 (pHP8080/pBluescript). 

^eOther clones determined to contain flbA (hp1041) based on the results of restriction analysis, PCR, and sequencing were as follows: pUDF109, -119, -137, -138, -141, -145, -149, -153, -156, and -158. 

^fCytosolic, pellet, and supernatant refer to crude extracts of the cytosol, the cell debris, and the extracellular supernatant, respectively. 

^gNA, not applicable.