Figure 3.

MLC₂₀ regulates the recruitment of c-Ab, cortactin, Pfn-1, and Abi1 to the cell edge. (A) Ctrl and MLC₂₀ KD cells were plated onto collagen-coated coverslips for 30 min followed by immunofluorescence and fluorescence analysis. MLC₂₀ KD attenuates the localization of c-Abl, cortactin (cort), Pfn-1, Abi1, and F-actin at the cell edge. The arrows point to the leading edge. Scale bar: 10 µm. (B) Illustration of quantitative analysis. An average of at least 5 line scans across each cell is used for analysis. Relative Intensity (RI) = Edge Intensity (EdI)/Cortex Intensity (CI). (C) Data are mean values of experiments from at least 20 cells for each group. Error bars indicate SD. One-way ANOVA was used for statistical analysis. ** p < 0.01; * p < 0.05.