Figure 5.

MLC₂₀ phosphorylation and actin polymerization regulate the recruitment of actin-regulatory proteins to the cell edge. (A) Myosin light chain kinase (MLCK) colocalizes with integrin β1 at the cell edge. Cells were immunostained for MLCK and integrin β1. The arrows point to the leading edge. Scale bar: 10 µm. (B) Cells were plated onto collagen-coated coverslips in the absence or presence of 1 µM ML7 or 10 nM latrunculin A (LAT-A) for 30 min. They were then stained for the indicated proteins or F-actin. Scale bar, 10 µm. Ctrl, control cells. The arrows point to the leading edges. (C) Data are mean values of experiments from at least 20 cells for each group. Error bars indicate SD. ** p < 0.01. One-way ANOVA was used for statistical analysis.