Figure 6.

Integrin β1 controls the positioning of MLCK at the tip of the protrusion. (A) Human airway smooth muscle (HASM) cells treated with control (Ctrl) siRNA or β1 siRNA were evaluated by immunoblot analysis. Data are mean values of experiments from five cultures from three donors. Error bars indicate SD. (B) β1 KD attenuates the positioning of MLCK and pMLC₂₀ at the cell edge. The arrows point to the leading edge. Scale bar: 10 µm. Data are mean values of experiments from at least 20 cells for each group. Error bars indicate SD. ** p < 0.01. The t-test was used for statistical analysis.